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Single-shot 20-fold Expansion Microscopy

Wang S*, Shin TW*, Yoder HB 2nd, McMillan RB, Su H, Liu Y, Zhang C, Leung KS, Yin P, Kiessling LL**, Boyden ES**; Nature Methods, 2024 doi:10.1038/s41592-024-02454-9. Online ahead of print.

 

In Vivo Optical Clearing of Mammalian Brain

Giovanni Talei Franzesi*, Ishan Gupta*, Ming Hu, Kiryl Piatkveich, Murat Yildirim, Jian-Ping Zhao, Minho Eom, Seungjae Han, Demian Park, Himashi Andaraarachchi, Zhaohan Li, Jesse Greenhagen, Amirul Muhammad Islam, Parth Vashishtha, Zahid Yaqoob, Nikita Pak, Alexander D. Wissner-Gross, Daniel Martin-Alarcon, Jonathan Veinot, Peter T. So, Uwe Kortshagen, Young-Gyu Yoon, Mriganka Sur**, Edward S. Boyden** 2024, bioRxiv 2024.09.05.611421; doi: https://doi.org/10.1101/2024.09.05.611421 ​​​​​

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Dense, Continuous Membrane Labeling and Expansion Microscopy Visualization of Ultrastructure in Tissues

Tay Won Shin, Hao Wang*, Chi Zhang*, Bobae An, Yangning Lu, Elizabeth Zhang, Xiaotang Lu, Emmanouil D Karagiannis, Jeong Seuk Kang, Amauche Emenari, Panagiotis Symvoulidis, Shoh Asano, Leanne Lin, Emma K Costa; IMAXT Grand Challenge Consortium; Adam H Marblestone, Narayanan Kasthuri, Li-Huei Tsai, Edward S Boyden 2024, bioRxiv 2024.03.07.583776; doi: https://doi.org/10.1101/2024.03.07.583776

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One-step nanoscale expansion microscopy reveals individual protein shapes

Shaib AH, Chouaib AA, Chowdhury R, Altendorf J, Mihaylov D, Zhang C, Krah D, Imani V, Spencer RKW, Georgiev SV, Mougios N, Monga M, Reshetniak S, Mimoso T, Chen H, Fatehbasharzad P, Crzan D, Saal KA, Alawieh MM, Alawar N, Eilts J, Kang J, Soleimani A, Müller M, Pape C, Alvarez L, Trenkwalder C, Mollenhauer B, Outeiro TF, Köster S, Preobraschenski J, Becherer U, Moser T, Boyden ES, Aricescu AR, Sauer M, Opazo F, Rizzoli SO; Nature Biotechnology, 2024, doi:10.1038/s41587-024-02431-9. 

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Improved immunostaining of nanostructures and cells in human brain specimens through expansion-mediated protein decrowding

Valdes PA, Yu CJ*, Aronson J*, Ghosh D, Zhao Y, An B, Bernstock JD, Bhere D, Felicella MM, Viapiano MS, Shah K, Chiocca EA**, Boyden ES** Science Translational Medicine 2023, 16(732):eabo0049.

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Imaging the voltage of neurons distributed across entire brains of larval zebrafish

Zeguan Wang*, Jie Zhang*, Panagiotis Symvoulidis, Wei Guo, Lige Zhang, Matthew A. Wilson, Edward S. Boyden (2023) bioRxiv 2023.12.15.571964; doi: https://doi.org/10.1101/2023.12.15.571964 

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Expansion microscopy using a single anchor molecule for high-yield multiplexed imaging of proteins and RNAs

Cui Y*, Yang G*, Goodwin DR, O'Flanagan CH, Sinha A, Zhang C, Kitko KE, Shin TW, Park D, Aparicio S; CRUK IMAXT Grand Challenge Consortium; Boyden ES, PLoS One 2023, 8(9):e0291506.

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Expansion Technologies received $500K NIH-SBIR grant to develop High-resolution 3D in situ Spatial Gene Expression Profiling Technology for Human Brain Specimens

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Revealing nanostructures in brain tissue via protein decrowding by iterative expansion microscopy

Deblina Sarkar*, Jinyoung Kang*, Asmamaw T. Wassie*, Margaret E. Schroeder, Zhuyu Peng, Tyler B. Tarr, Ai-Hui Tang, Emily D. Niederst, Jennie Z. Young, Hanquan Su, Demian Park, Peng Yin, Li-Huei Tsai**, Thomas A. Blanpied** & Edward S. Boyden**; Nature Biomedical Engineering, 2022 6:1057–1073 

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A highly homogeneous polymer composed of tetrahedron-like monomers for high-isotropy expansion microscopy 

Gao R, Yu C.J*, Gao L, Piatkevich K.D, Neve R.L, Munro J.B, Upadhyayula S, Boyden E.S Nature Nanotechnology, 2021,  16:698–707

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Expansion Sequencing: Spatially Precise In Situ Transcriptomics in Intact Biological Systems

Alon S*, Goodwin D.R*, Sinha A*, Wassie A.T*, Chen F*, Daugharthy E.R**, Bando Y, Kajita A, Xue A.G, Marrett K, Prior R, Cui Y, Payne A.C, Yao C.C, Suk H.J, Wang R, Yu C.J, Tillberg P, Reginato P, Pak N, Liu S, Punthambaker S, Iyer E.P.R, Kohman R.E, Miller J.A, Lein E.S, Lako A, Cullen N, Rodig S, Helvie K, Abravanel D.L, Wagle N, Johnson B.E, Klughammer J, Slyper M, Waldman J, Jané-Valbuena J, Rozenblatt-Rosen O, Regev A; IMAXT Consortium, Church G.M***+, Marblestone A.H***, Boyden E.S***;  Science, 2021, 371(6528) : eaax2656

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Expansion microscopy of C. elegans

Yu C.J, Barry N.C, Wassie A.T, Sinha A, Bhattacharya A, Asano S, Zhang C, Chen F, Hobert O, Goodman M.B, Haspel G, Boyden E.S eLife 2020, 9:e46249.​

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Expansion Microscopy: Principles and Uses in Biological Research

Asmamaw T. Wassie, Yongxin Zhao and Edward S. Boyden; Nature Methods, 2019, 16, 33–41

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Expansion Microscopy of Zebrafish for Neuroscience and Developmental Biology Studies

Limor Freifeld, Iris Odstrcil, Dominique Förster, Alyson Ramirez, James A. Gagnon, Owen Randlett, Emma K. Costa, Shoh Asano, Orhan T. Celiker, Ruixuan Gao, Daniel A. Martin-Alarcon, Paul Reginato, Cortni Dick, Linlin Chen, David Schoppik, Florian Engert, Herwig Baier, and Edward S. Boyden; PNAS, 2017,  114 (50), E10799-E10808.​​​