A highly homogeneous polymer composed of tetrahedron-like monomers for high-isotropy expansion microscopy
Gao R*, Yu C.J*, Gao L, Piatkevich K.D, Neve R.L, Munro J.B, Upadhyayula S, Boyden E.S Nature Nanotechnology, 2021, 16:698–707
Expansion Sequencing: Spatially Precise In Situ Transcriptomics in Intact Biological Systems
Alon S*, Goodwin D.R*, Sinha A*, Wassie A.T*, Chen F*, Daugharthy E.R**, Bando Y, Kajita A, Xue A.G, Marrett K, Prior R, Cui Y, Payne A.C, Yao C.C, Suk H.J, Wang R, Yu C.J, Tillberg P, Reginato P, Pak N, Liu S, Punthambaker S, Iyer E.P.R, Kohman R.E, Miller J.A, Lein E.S, Lako A, Cullen N, Rodig S, Helvie K, Abravanel D.L, Wagle N, Johnson B.E, Klughammer J, Slyper M, Waldman J, Jané-Valbuena J, Rozenblatt-Rosen O, Regev A; IMAXT Consortium, Church G.M***+, Marblestone A.H***, Boyden E.S***+ Science, 2021, 371(6528) : eaax2656
Expansion Microscopy for Beginners: Visualizing Microtubules in Expanded Cultured HeLa Cells
Zhang C*, Kang J.*, Asano S.M, Gao R, Boyden E.S Current Protocols in Neuroscience, 2020, 92(1):e96
Expansion microscopy of C. elegans
Yu C.J, Barry N.C, Wassie A.T, Sinha A, Bhattacharya A, Asano S, Zhang C, Chen F, Hobert O, Goodman M.B, Haspel G, Boyden E.S eLife 2020, 9:e46249.
Nanoscale imaging of clinical specimens using conventional and rapid expansion pathology
Bucur O*, Fu F*, Calderon M, Mylvaganam G.H, Ly N.L, Day J, Watkin S, Walker B.D, Boyden E.S**, Zhao Y** Nature Protocols , 2020, 15:1649-1672
Expansion Microscopy: Principles and Uses in Biological Research
Asmamaw T. Wassie, Yongxin Zhao and Edward S. Boyden; Nature Methods, 2019, 16, 33–41
Expansion Microscopy: Protocols for Imaging Proteins and RNA in Cells and Tissues
Shoh M. Asano Ruixuan Gao Asmamaw T. Wassie Paul W. Tillberg Fei Chen Edward S. Boyden; Current Protocols in Cell Biology, 2018,
Expansion microscopy: enabling single cell analysis in intact biological systems
Shahar Alon Grace H. Huynh Edward S. Boyden; The FEBS Journal, 2018,
Expansion microscopy: development and neuroscience applications
Emmanouil D Karagiannis and Edward SBoyden; Current Opinion in Neurobiology, 2018, 50, 56-63.
Expansion Microscopy of Zebrafish for Neuroscience and Developmental Biology Studies
Limor Freifeld, Iris Odstrcil, Dominique Förster, Alyson Ramirez, James A. Gagnon, Owen Randlett, Emma K. Costa, Shoh Asano, Orhan T. Celiker, Ruixuan Gao, Daniel A. Martin-Alarcon, Paul Reginato, Cortni Dick, Linlin Chen, David Schoppik, Florian Engert, Herwig Baier, and Edward S. Boyden; PNAS, 2017, 114 (50), E10799-E10808.
Feasibility of 3D Reconstruction of Neural Morphology Using Expansion Microscopy and Barcode-Guided Agglomeration
Young-Gyu Yoon, Peilun Dai, Jeremy Wohlwend, Jae-Byum Chang, Adam H. Marblestone and Edward S. Boyden; Frontiers in Computational Neuroscience, 2017, 11:97.
Q&A: Expansion microscopy
Ruixuan Gao, Shoh M. Asano, and Edward S. Boyden; BMC Biology, 2017, 15:50.
Tools for Mapping and Repairing Brain
Prof. Ed Boyden presents advanced super-resolution imaging platform at 2017 - Future of Genomic Medicine. March 03, 2017
Expansion mini-microscopy: An enabling alternative in point-of-care diagnostics
Yu Shrike Zhang, Grissel Trujillo-de Santiago, Mario Moisés Alvarez, Steven J. Schiff, Edward S. Boyden, Ali Khademhosseini; Current Opinion in Biomedical Engineering, 2017, 1, 45-53.
Nanoscale imaging of clinical specimens using pathology-optimized expansion microscopy
Yongxin Zhao, Octavian Bucur, Humayun Irshad, Fei Chen, Astrid Weins, Andreea L Stancu, Eun-Young Oh, Marcello DiStasio, Vanda Torous, Benjamin Glass, Isaac E Stillman, Stuart J Schnitt, Andrew H Beck & Edward S Boyden; Nature Biotechnology, 2017, 35, 957–964.
Iterative expansion microscopy
Jae-Byum Chang, Fei Chen, Young-Gyu Yoon, Erica E Jung, Hazen Babcock, Jeong Seuk Kang, Shoh Asano, Ho-Jun Suk, Nikita Pak, Paul W Tillberg, Asmamaw T Wassie, Dawen Cai & Edward S Boyden; Nature Methods, 2017, 14, 593–599
A new way to study the brain's invisible secrets
Prof. Ed Boyden TED talk on how responsive polymers inspired a revolution in super-resolution imaging. Aug 29, 2016
Nanoscale imaging of RNA with expansion microscopy
Fei Chen, Asmamaw T Wassie, Allison J Cote, Anubhav Sinha, Shahar Alon, Shoh Asano, Evan R Daugharthy, Jae-Byum Chang, Adam Marblestone, George M Church, Arjun Raj & Edward S Boyden; Nature Methods, 2016, 13, 679–684. (Highlighted as Method to Watch in 2016 Nature Methods)
Protein-retention expansion microscopy of cells and tissues labeled using standard fluorescent proteins and antibodies
Paul W Tillberg, Fei Chen, Kiryl D Piatkevich, Yongxin Zhao, Chih-Chieh (Jay) Yu, Brian P English, Linyi Gao, Anthony Martorell, Ho-Jun Suk, Fumiaki Yoshida, Ellen M DeGennaro, Douglas H Roossien, Guanyu Gong, Uthpala Seneviratne, Steven R Tannenbaum, Robert Desimone, Dawen Cai & Edward S Boyden; Nature Biotechnology, 2016, 34, 987–992.
Expansion microscopy
Fei Chen, Paul W. Tillberg, Edward S. Boyden; Science, 2015, 347 (6221), 543-548.