Single-shot 20-fold Expansion Microscopy

Wang S*, Shin TW*, Yoder HB 2nd, McMillan RB, Su H, Liu Y, Zhang C, Leung KS, Yin P, Kiessling LL**, Boyden ES** (2024) , Nature Methods, doi:10.1038/s41592-024-02454-9. Online ahead of print.

 

In Vivo Optical Clearing of Mammalian Brain

Giovanni Talei Franzesi*, Ishan Gupta*, Ming Hu, Kiryl Piatkveich, Murat Yildirim, Jian-Ping Zhao, Minho Eom, Seungjae Han, Demian Park, Himashi Andaraarachchi, Zhaohan Li, Jesse Greenhagen, Amirul Muhammad Islam, Parth Vashishtha, Zahid Yaqoob, Nikita Pak, Alexander D. Wissner-Gross, Daniel Martin-Alarcon, Jonathan Veinot, Peter T. So, Uwe Kortshagen, Young-Gyu Yoon, Mriganka Sur**, Edward S. Boyden** (2024), bioRxiv 2024.09.05.611421; doi: https://doi.org/10.1101/2024.09.05.611421 ​​​

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Tools for Analyzing, Repairing, and Simulating the Brain

Boyden, E.S. (2024), Neurotechnology and its Societal and Ethical Implications, Workshop at Casina Pio IV, 13-14 December 2023, Pontifical Academy of Sciences

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Dense, Continuous Membrane Labeling and Expansion Microscopy Visualization of Ultrastructure in Tissues

Tay Won Shin, Hao Wang*, Chi Zhang*, Bobae An, Yangning Lu, Elizabeth Zhang, Xiaotang Lu, Emmanouil D Karagiannis, Jeong Seuk Kang, Amauche Emenari, Panagiotis Symvoulidis, Shoh Asano, Leanne Lin, Emma K Costa; IMAXT Grand Challenge Consortium; Adam H Marblestone, Narayanan Kasthuri, Li-Huei Tsai, Edward S Boyden (2024), bioRxiv 2024.03.07.583776; doi: https://doi.org/10.1101/2024.03.07.583776

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Improved immunostaining of nanostructures and cells in human brain specimens through expansion-mediated protein decrowding

Valdes PA, Yu CJ*, Aronson J*, Ghosh D, Zhao Y, An B, Bernstock JD, Bhere D, Felicella MM, Viapiano MS, Shah K, Chiocca EA**, Boyden ES** (2024), Science Translational Medicine 16(732):eabo0049.

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Imaging the voltage of neurons distributed across entire brains of larval zebrafish

Zeguan Wang*, Jie Zhang*, Panagiotis Symvoulidis, Wei Guo, Lige Zhang, Matthew A. Wilson, Edward S. Boyden (2023) bioRxiv 2023.12.15.571964; doi: https://doi.org/10.1101/2023.12.15.571964 

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Temporally multiplexed imaging of dynamic signaling networks in living cells

Qian Y, Celiker OT, Wang Z, Guner-Ataman B, Boyden ES (2023), Cell 186(25):5656-5672.

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Optogenetic Control of Neural Activity: the Biophysics of Microbial Rhodopsins in Neuroscience

Piatkevich KD, Boyden ES (2023) , Quarterly Review of Biophysics 13:1-81.

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Expansion microscopy using a single anchor molecule for high-yield multiplexed imaging of proteins and RNAs

Cui Y*, Yang G*, Goodwin DR, O'Flanagan CH, Sinha A, Zhang C, Kitko KE, Shin TW, Park D, Aparicio S; CRUK IMAXT Grand Challenge Consortium; Boyden ES (2023) , PLoS One 18(9):e0291506.

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A highly homogeneous polymer composed of tetrahedron-like monomers for high-isotropy expansion microscopy 

Gao R*, Yu C.J*, Gao L, Piatkevich K.D, Neve R.L, Munro J.B, Upadhyayula S, Boyden E.S Nature Nanotechnology, 2021,  16:698–707

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Expansion Sequencing: Spatially Precise In Situ Transcriptomics in Intact Biological Systems

Alon S*, Goodwin D.R*, Sinha A*, Wassie A.T*, Chen F*, Daugharthy E.R**, Bando Y, Kajita A, Xue A.G, Marrett K, Prior R, Cui Y, Payne A.C, Yao C.C, Suk H.J, Wang R, Yu C.J, Tillberg P, Reginato P, Pak N, Liu S, Punthambaker S, Iyer E.P.R, Kohman R.E, Miller J.A, Lein E.S, Lako A, Cullen N, Rodig S, Helvie K, Abravanel D.L, Wagle N, Johnson B.E, Klughammer J, Slyper M, Waldman J, Jané-Valbuena J, Rozenblatt-Rosen O, Regev A; IMAXT Consortium, Church G.M***+, Marblestone A.H***, Boyden E.S***+ Science, 2021, 371(6528) : eaax2656

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Expansion microscopy of C. elegans

Yu C.J, Barry N.C, Wassie A.T, Sinha A, Bhattacharya A, Asano S, Zhang C, Chen F, Hobert O, Goodman M.B, Haspel G, Boyden E.S eLife 2020, 9:e46249.​

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Expansion Microscopy: Principles and Uses in Biological Research

Asmamaw T. Wassie, Yongxin Zhao and Edward S. Boyden; Nature Methods, 2019, 16, 33–41

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Expansion Microscopy: Protocols for Imaging Proteins and RNA in Cells and Tissues

Shoh M. Asano  Ruixuan Gao  Asmamaw T. Wassie  Paul W. Tillberg  Fei Chen  Edward S. Boyden; Current Protocols in Cell Biology, 2018, 

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Expansion Microscopy of Zebrafish for Neuroscience and Developmental Biology Studies

Limor Freifeld, Iris Odstrcil, Dominique Förster, Alyson Ramirez, James A. Gagnon, Owen Randlett, Emma K. Costa, Shoh Asano, Orhan T. Celiker, Ruixuan Gao, Daniel A. Martin-Alarcon, Paul Reginato, Cortni Dick, Linlin Chen, David Schoppik, Florian Engert, Herwig Baier, and Edward S. Boyden; PNAS, 2017,  114 (50), E10799-E10808.​